Mutant of Bordetella pertussis which lacks ability to produce filamentous hemagglutinin
نویسندگان
چکیده
منابع مشابه
Antigenic analysis of Bordetella pertussis filamentous hemagglutinin with phage display libraries and rabbit anti-filamentous hemagglutinin polyclonal antibodies.
Although substantial advancements have been made in the development of efficacious acellular vaccines against Bordetella pertussis, continued progress requires better understanding of the antigenic makeup of B. pertussis virulence factors, including filamentous hemagglutinin (FHA). To identify antigenic regions of FHA, phage display libraries constructed by using random fragments of the 10-kbp ...
متن کاملSubcutaneous administration of a fusion protein composed of pertussis toxin and filamentous hemagglutinin from Bordetella pertussis induces mucosal and systemic immune responses
Objective(s): After decades of containment, pertussis disease, caused by Bordetella pertussis seems to be re-emerging and still remains a major cause of reported vaccine-preventable deaths worldwide. The current licensed whole-cell vaccines display reactogenicity while acellular vaccines are expensive and do not induce Th1-type immune responses that are required for optimum protection against t...
متن کاملexpression, purification and characterization of three overlapping immunodominant recombinant fragments from bordetella pertussis filamentous hemagglutinin
background: filamentous hemagglutinin (fha) is one of the most important immunoprotective antigens of bordetella pertussis (b.pertussis) and a major component of the acellular pertussis vaccine. in the present study, three overlapping recombinant fragments from the immunodominant region of fha were produced and their immunogenicity was investigated. methods: three overlapping coding sequences o...
متن کاملCloning, partial sequence, expression, and antigenic analysis of the filamentous hemagglutinin gene of Bordetella pertussis.
The gene coding for the filamentous hemagglutinin (FHA), one of the main factors involved in mediating adherence of Bordetella pertussis to ciliated host cells, was cloned in Escherichia coli, and the 3,500-base-pair nucleotide sequence encoding the amino-terminal region was determined. Molecular cloning, together with the characterization of recombinant FHA-related proteins produced in E. coli...
متن کاملCloning of the filamentous hemagglutinin of Bordetella pertussis and its expression in Escherichia coli.
Bordetella pertussis UT25 DNA was cloned into the kanamycin resistance gene of cosmid pCP13 to construct a genomic library in Escherichia coli LE392. One clone containing plasmid pDB441 expressed the filamentous hemagglutinin (FHA) as identified by protein immunoblots with the use of rabbit anti-B. pertussis antiserum, rabbit anti-FHA antiserum, and a monoclonal antibody to FHA. FHA is a protei...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1982
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.35.3.1018-1023.1982